Essential Biochemistry - Genetic Engineering (Section 2)
1
Which of the following is not an application of genetic engineering?
Trả lời(D)
Development of new antibiotics
2
What is the role of DNA ligase in recombinant DNA technology?
Trả lời(B)
Joining DNA fragments together.
3
What is the purpose of a selectable marker in a cloning vector?
Trả lời(A)
To allow for the identification of cells that have taken up the vector.
4
What is the key advantage of using plasmids as cloning vectors?
Trả lời(A)
They are small and easy to manipulate.
5
Polymerase chain reaction (PCR) is a technique for
Trả lời(A)
Amplification of DNA in vitro
6
Which of the following techniques is used to amplify DNA fragments?
7
What is the purpose of the extension step in PCR?
Trả lời(C)
To synthesize new DNA strands.
8
Which of the following is NOT a common use of PCR?
Trả lời(C)
Genetically modifying an organism
9
What is the role of a selectable marker in a cloning vector?
Trả lời(B)
To allow for the identification of cells that have taken up the vector.
10
What is the function of a promoter in a cloning vector?
Trả lời(A)
To signal the start of transcription.
11
What is the name of the process where foreign DNA is introduced into a bacterial cell?
12
What is the primary difference between a genomic DNA library and a cDNA library?
Trả lời(A)
Genomic libraries contain all DNA sequences, while cDNA libraries contain only coding sequences.
13
Which of the following is NOT a characteristic of a cloning vector?
14
Which one of the following enzyme is required for PCR?
15
What is the role of ligase in recombinant DNA technology?
16
Which of the following is not a step in recombinant DNA technology?
Trả lời(D)
Hybridization of the gene of interest with mRNA
17
What is the function of a probe in Southern blotting?
Trả lời(B)
To identify a specific DNA sequence
18
Which of the following are key components in the process of creating recombinant DNA?
19
What type of analysis is used to detect the presence of specific DNA sequences in a sample?
20
In genetic engineering, which of the following enzymes is used to join DNA fragments?
21
The main purpose of using a cloning vector is to:
22
In gel electrophoresis, DNA fragments move toward which electrode?
23
What is the principle behind the use of RFLP analysis?
Trả lời(A)
Differences in DNA sequence can lead to variations in the size of DNA fragments after digestion with restriction enzymes.
24
Which of the following is an example of a commonly used restriction enzyme?
25
Which of the following is used to cut DNA at specific locations?
26
Which technique is used to analyze RNA molecules?
27
Restriction endonuclease has the following characteristics, except:
28
What does cDNA stand for?
29
Most commonly used cloning vectors are
30
What is the role of a vector in recombinant DNA technology?
