Essential Biochemistry - Genetic Engineering (Section 1)
1
A scientist wants to identify a specific gene within a large genome. Which technique would be most suitable?
2
What does the term 'transgenic' mean?
คำตอบ(A)
An organism with a modified genome
3
Which of the following is a benefit of using recombinant DNA technology?
4
What is an advantage of using PCR?
คำตอบ(C)
It can amplify a specific DNA sequence
5
Which technique is used to detect specific RNA molecules?
6
Which of the following is NOT required for PCR?
7
What process does a restriction enzyme perform?
8
What is the purpose of Taq polymerase in PCR?
คำตอบ(C)
To synthesize new DNA strands
9
What does RFLP stand for?
คำตอบ(B)
Restriction Fragment Length Polymorphism
10
Which blotting technique is used to analyze proteins?
11
คำตอบ(B)
A collection of cDNA clones representing the expressed genes in a cell
12
What is the role of a selectable marker in a cloning vector?
คำตอบ(B)
To identify cells that have successfully taken up the vector
13
Which is NOT a common application of recombinant DNA technology?
14
What is the primary purpose of using restriction enzymes in recombinant DNA technology?
คำตอบ(B)
To cut DNA at specific sequences
15
What is the main function of a cloning vector?
คำตอบ(B)
To amplify a DNA fragment
16
Cloning a technique developed for amplifying the quantity of DNA in vitro
17
Which techniques would be useful to test the presence of a specific DNA sequence?
18
What is the purpose of a selectable marker in a cloning vector?
คำตอบ(B)
To identify cells containing the vector
19
Restriction endonucleases are enzymes:
คำตอบ(B)
Which clears DNA at specific sequences
20
Restriction endonuclease has the following characteristics, except:
21
The process of joining of DNA fragments to form a hybrid molecule is called:
22
What is the function of DNA ligase?
23
What is the role of a primer in PCR?
คำตอบ(C)
To initiate DNA synthesis
24
Which of the following techniques is used to separate DNA fragments based on their size?
25
What is a main goal of genetic engineering?
คำตอบ(B)
To alter the genetic makeup of an organism
26
Which technique is used to amplify a specific DNA sequence?
27
Which one of the following is not an enzyme?
28
Most commonly used cloning vectors are:
29
Which of the following is a key component of a cloning vector?
30
What is the role of a selectable marker in a cloning vector?
คำตอบ(B)
To identify cells that have taken up the vector.
31
In recombinant DNA technology, what does the term 'vector' refer to?
คำตอบ(D)
The carrier molecule (e.g., plasmid) used to introduce foreign DNA into a host cell
32
Which of the following techniques can be used to detect the presence of a specific protein?
33
What is the process of introducing foreign DNA into a host cell called?
34
Which one of the following enzyme is required for PCR?
35
คำตอบ(A)
The use of genes to treat diseases
36
Which of the following is NOT a step in the process of creating recombinant DNA?
คำตอบ(D)
Using a ribosome to synthesize the protein
37
Southern blot is a technique used for analysis of
38
คำตอบ(C)
An extrachromosomal DNA molecule
39
What is the purpose of a DNA library?
40
What is the role of a promoter region in a gene?
คำตอบ(B)
To signal the start of transcription
41
Restriction endonucleases are enzymes
คำตอบ(B)
Which shares DNA at specific sequences
42
What is the primary goal of gene therapy?
คำตอบ(A)
To replace faulty genes with healthy genes
43
What is the role of the annealing step in PCR?
คำตอบ(B)
To allow primers to bind to the DNA template
44
Which enzyme is used to seal the gaps between DNA fragments during cloning?
45
Which of the following is a technique used for gene cloning?
46
What is a transgenic organism?
คำตอบ(A)
An organism that has a foreign gene introduced into its genome.
47
What is the purpose of gel electrophoresis in recombinant DNA technology?
คำตอบ(B)
To separate DNA fragments by size
48
What is the role of reverse transcriptase in cDNA library construction?
49
What is the role of the primer in PCR?
คำตอบ(B)
To provide a starting point for DNA polymerase
50
What is the name of the process by which a virus transfers genetic material to a bacterium?
