Quick Biochemistry - Proteins And Protein Metabolism (Section 14)
1
When using a pure protein to generate antibodies, which method will show a single distinct peak?
Answer
(D)
Immuno electrophoresis
2
Which method utilizes the unique pH at which a protein carries no net electrical charge?
Answer
(A)
Isoelectric focussing
3
Which method separates proteins based on their differing net charges at a specific pH?
Answer
(B)
lon exchange chromatography
4
What term describes the ratio of a substance's travel distance to the solvent's travel distance?
Answer
(C)
Rf value
5
What is the term for the migration of charged particles in response to an electrical field?
Answer
(D)
Electrophoresis
6
Which material is an anion exchange resin bound to a cellulose base?
Answer
(A)
DEAE cellulose
7
Which material is a cation exchange resin bound to a cellulose base?
Answer
(A)
CM-cellulose
8
Which technique can be used to separate molecules based on their size and shape for protein purification?
Answer
(B)
Gel filtration chromatography
9
What materials are often used in adsorption chromatography for protein separation?
Answer
(C)
Calcium phosphate gel alumina gel and hydroxy apatite
10
What is the process where the solubility of most proteins decreases at high salt concentrations?
Answer
(B)
Salting out process
11
Which of the following is a precursor in the formation of a specific compound?
Answer
(A)
Compound A
12
Which diuretic does NOT act by interfering with carbonic anhydrase?
Answer
(D)
Diuretic D
13
What are proteins built from?
Answer
(C)
Building Block C
14
Which of the following is NOT capable of rotating polarized light?
Answer
(D)
Molecule D
15
What are biochemical reaction accelerators made of?
Answer
(C)
Substance C
16
What biological process is directly regulated by Insulin?
Answer
(B)
Process B
17
Which type of protein plays a crucial role in the body's defense system?
Answer
(B)
Protein B
18
At what pH level does an amino acid have a balanced charge?
Answer
(C)
pH 7.0
19
What process utilizes the isoelectric point of amino acids?
Answer
(B)
Process B
20
The Xanthoproteic test identifies proteins with what component?
Answer
(C)
Component C
21
Which test yields a positive result for all α-amino acids?
Answer
(D)
A test utilizing a specific chemical compound
22
What method is used to determine the N-terminal amino acids in a polypeptide?
Answer
(A)
A reaction named after a scientist
23
Which amino acid gives a positive reaction with Million's test?
Answer
(C)
An amino acid containing a phenol group
24
Which reagent produces a positive result when reacting with the indole group of tryptophan?
Answer
(A)
A compound containing a glyoxylic acid
25
The guanidine group of arginine gives a positive test with which reagent?
Answer
(B)
A reagent named after a scientist
26
Which reagent is used to detect the thiol group of cysteine and results in a red color?
Answer
(C)
A compound using a nitroprusside
27
Which condition is caused by a protein deficiency?
Answer
(D)
A condition characterized by protein and calorie deficiency
28
Which of the following is a plant-based source of protein?
Answer
(B)
A plant known for its use in traditional medicine
29
What enzyme catalyzes the conversion of oxaloacetate to aspartic acid?
Answer
(C)
An enzyme involved in transferring amino groups
30
A lack of biotin leads to a decrease in which process?
Answer
(D)
The synthesis of fatty acids
31
What substance serves as the starting material for bile salts, sex hormones, and vitamin D production?
Answer
(B)
Cholesterol
32
What is the term for fatty acids that contain double or triple bonds between carbon atoms?
Answer
(B)
Essential fatty acids
33
Which of the following is specifically detected by the Biuret test?
Answer
(A)
Two peptide linkage
34
Where is the majority of calcium found in the body, and what term describes the calcium in soft tissue and blood?
Answer
(C)
Physiological blood
35
How is calcium bound to protein described, versus its presence in salt form?
Answer
(A)
Bound
36
Which of the following ions plays a role in facilitating the transfer of phosphate from ATP to pyruvic acid by enzymes?
Answer
(D)
Potassium
37
How many main categories does the International Enzyme Commission use to classify enzymes?
Answer
(B)
Six classes
38
What biological molecule is the subject of the Michaelis-Menten equation, used to study its concentration effects?
Answer
(B)
Enzyme
39
What term is used to describe the pH level at which an enzyme exhibits its highest level of activity?
Answer
(B)
Optimum pH
40
What is the process called where proteins are broken down into amino acids, carbohydrates into glucose, and lipids into fatty acids?
Answer
(C)
Catabolism
41
In the process of glucose metabolism without oxygen, the released energy is called:
Answer
(D)
Anaerobic fermentation
42
A lack of urea cycle enzymes leads to a buildup of specific compounds in the liver. This results in increased levels of what substance in the bloodstream?
Answer
(C)
Ammonia
43
The condition of elevated trytophan levels in the blood is referred to as:
Answer
(D)
Hartnup's disease
44
What is the primary function of lymphocytes in the body?
Answer
(C)
Antibody production
45
Blood platelets contain an enzyme essential for blood clotting. This enzyme is called:
Answer
(D)
Thrombokinase
46
When pentoses react with a strong mineral acid, the resulting cyclic aldehyde is known as:
Answer
(D)
Furfural
47
At what electrical state is a protein at its isoelectric pH?
Answer
(A)
Neutral
48
Approximately how much hemoglobin is present in a healthy 70 kg adult male?
Answer
(D)
70 g
49
Which of the following is NOT found in pancreatic juice?
Answer
(C)
Cholecystokinin
50
Which enzyme is responsible for digesting the milk protein in the stomach of an adult?
