Quick Biochemistry - Nucleic Acids (Section 6)
1
Which of the following is NOT a characteristic of orotic aciduria?
Answer(A)
Enhanced production of pyrimidine nucleotides
2
A genetic lack of adenosine deaminase leads to
3
A total lack of hypoxanthine guanine phospharibosyl transferase results in
4
Increased excretion of orotic acid in urine can be caused by a deficiency in
Answer(D)
Any of the options listed
5
Which of the following is NOT a typical symptom of Lesch-Nyhan syndrome?
6
A hereditary deficiency in purine nucleoside phosphorylase is most likely to cause
7
Deoxyribonucleotides are produced through the reduction of
Answer(C)
Ribonucleoside diphosphates
8
What other compound can orotate phosphoribosyl transferase use?
9
Why is gout less common in mammals other than higher primates?
10
Low levels of uric acid can occur in individuals with
Answer(A)
Xanthine oxidase deficiency
11
The process of creating a DNA copy is also termed
12
The method by which DNA is replicated is described as
13
In what direction is new DNA generally assembled
14
In the DNA synthesis process, what usually comes before
15
Short segments found in DNA replication consist of
16
Which of the following molecules has its assembly that results in Okazaki fragments
17
When the replication fork appears
Answer(B)
One strand is made continuously, the other with breaks
18
About how many units make up one Okazaki fragment
19
Which enzyme is responsible for creating the RNA primer
20
In RNA, what base pairs with adenine
21
In the process of duplication, where does the original DNA strand separate?
22
In DNA replication, what molecule initiates the separation of the double helix?
23
To separate the double-stranded DNA, what is needed?
24
What action do helicase and DnaB protein perform?
Answer(C)
Unwinding DNA and requires ATP
25
After the DNA strands separate, how are they kept apart?
Answer(A)
Single strand binding protein
26
What enzyme adds deoxyribonucleotides to the RNA primer?
Answer(C)
DNA polymerase III holoenzyme
27
Which enzyme replaces the RNA primer's ribonucleotides with deoxyribonucleotides?
28
What is used to join DNA fragments together?
29
What introduces negative supercoils into the DNA structure?
30
Which of the following contains reverse transcriptase activity in eukaryotic cells?
31
What function does the DNA polymerase III holoenzyme perform?
Answer(D)
3'ā5' Exonuclease and polymerase activities
32
What is a characteristic function of DNA polymerase I?
33
What is the role of the 3'ā5' Exonuclease activity of DNA polymerase I?
Answer(C)
Corrects errors in replication
34
Which of the following statements about RNA-dependent DNA polymerase is incorrect?
Answer(D)
It is present in all the viruses
35
What reaction does reverse transcriptase facilitate?
36
What type of DNA does the DNA A protein specifically bind to?
Answer(B)
Negatively supercoiled DNA
37
What reaction does DNA topoisomerase I of E. coli catalyze?
Answer(A)
Relaxation of negatively supercoiled DNA
38
During which phase of the mammalian cell cycle does DNA synthesis take place?
39
At what temperature does the melting of DNA occur?
Answer(D)
Native double helical DNA is denatured
40
What factor increases the melting temperature of DNA?
41
The factor that enhances the buoyant density of DNA is related to its
Answer(B)
Guanine and Cytosine base composition
42
The ratio of Guanine and Cytosine to Adenine and Thymine within DNA can be calculated by examining its
43
Mitochondria in certain cell types contain some amount of DNA in
44
Which characteristic is observed in satellite DNA?
Answer(A)
Highly recurring sequences
45
What is the process of creating RNA using a DNA blueprint called?
46
In which direction is RNA generally synthesized?
Answer(A)
Five prime to three prime
47
DNA-dependent RNA polymerase has how many protein subunits?
48
For effective operation, DNA-dependent RNA polymerase requires
49
Which element identifies the transcription starting point?
50
The location where transcription concludes is determined by
