Essential Chemistry for IGCSE - Particles And Purification (Section 4 - No. 21)
What would happen if the sample spot was placed below the solvent level in paper chromatography?
The sample will dissolve and run up the paper
The solvent will dissolve the paper.
The sample will dissolve into the solvent, and the separation cannot occur.
Nothing will happen
Explanation
If the sample is below the solvent level, it will dissolve directly into the solvent, and the separation won't happen.
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