Essential Biochemistry - Laboratory Investigation Techniques (Section 2)

1
The separation depends on the relative behaviour of molecules to
Answer
(C)
Both A and B
2
What is a cofactor?
Answer
(B)
A non-protein molecule that helps an enzyme
3
What is the function of a chaperone protein?
Answer
(C)
To assist in protein folding
4
The separation depends on the relative behaviour of the molecules in a mixture to associate more strongly with
Answer
(B)
the stationary phase
5
What is the primary function of mRNA?
Answer
(A)
To carry genetic information from DNA to ribosomes
6
Which of the following techniques can be used in small laboratories?
Answer
(A)
ELISA
7
Which of the following is a type of secondary structure in proteins?
Answer
(C)
Alpha helix
8
Which of the following is a purine base found in DNA and RNA?
Answer
(C)
Guanine
9
What type of reaction typically occurs when a molecule is broken down with the addition of water?
Answer
(B)
Hydrolysis
10
Which of the following can be detected by ELISA?
Answer
A
B
C
11
Which molecule is the primary source of energy in cells?
Answer
(A)
Glucose
12
What is the role of ATP synthase?
Answer
(B)
To synthesize ATP
13
Which of the following is a characteristic of competitive enzyme inhibitors?
Answer
(C)
They increase the Km
14
What type of bond is formed between two amino acids?
Answer
(B)
Peptide
15
What is the role of the enzyme amylase?
Answer
(C)
Break down carbohydrates
16
The technique which depends on the antigen and the antibody is
Answer
(C)
Both A and B
17
What is the main function of the hormone insulin?
Answer
(B)
Decrease blood glucose levels
18
Which of the following is a technique used in ELISA?
Answer
(D)
Enzyme
19
What is the role of ATP in biological systems?
Answer
(B)
Energy transport
20
The application of RIA and ELISA is
Answer
(D)
All of the above
21
Which of the following is used for clinical application of electrophoresis?
Answer
(D)
All of the above
22
Which of the following molecules is a sterol?
Answer
(B)
Cholesterol
23
Which of the following is used as a label in ELISA?
Answer
(D)
Enzyme
24
What type of bond links amino acids together in a protein?
Answer
(B)
Peptide bond
25
Which of the following are the basic units of a colorimeter?
Answer
(A)
A light source, a cuvette, and a photodetector
26
What molecule is the final electron acceptor in the electron transport chain?
Answer
(B)
Oxygen
27
In the clinical laboratory for the identification of major amino acids and drugs in urine or serum.
Answer
(D)
All of the above
28
In paper chromatography, the solvent system provides
Answer
(A)
The mobile phase
29
What is the most abundant type of lipid in cell membranes?
Answer
(C)
Phospholipids
30
For which analysis colorimetry is used in the hospital?
Answer
(D)
All of the above
31
The most used technique to identify or quantify the antigen or antibody is
Answer
(D)
All of the above
32
Which of the following is a product of the Krebs cycle?
Answer
(C)
ATP
33
Inhibition of a certain pathway may be due to?
Answer
A
B
C
D
34
What does 'deamination' refer to?
Answer
(A)
Removal of an amino group
35
In radioimmunoassay, the sample is
Answer
(B)
Labeled with a radioactive isotope
36
Which of the following is a pyrimidine base found in DNA?
Answer
(C)
Cytosine
37
The ratio of the distance travelled by the solute to the distance travelled by the solvent is represented by
Answer
(C)
Both A and B
38
Chromatography is a process for separation of components in a solution by differences in migration rate as the solution moves
Answer
(A)
Mobile phase
39
What type of enzyme is responsible for breaking down proteins?
Answer
(C)
Protease
40
Which of the following can be measured using colorimetry?
Answer
A
B
D
41
The method is applicable for the separation of carbohydrates, proteins, peptides, sterol acids, vitamins, steroids, vaccines like fragile viruses and drugs.
Answer
(B)
HPLC
42
What is the basic structural unit of a carbohydrate?
Answer
(C)
Monosaccharide
43
The energy generated by the detector in flame photometry is measured by
Answer
(A)
Galvanometer
44
ELISA is a technique for determining the presence of
Answer
(C)
Both A and B
45
The principle of spectrophotometry is based on
Answer
(A)
Beer-Lambert's Law
46
What type of reaction is the breakdown of ATP to ADP and inorganic phosphate?
Answer
(A)
Hydrolysis
47
Which of the following is often used as the stationary phase in paper chromatography?
Answer
(A)
Filter paper
48
In paper chromatography, the relative of a sample are separated by differences in their relative solubilities between
Answer
(B)
Liquid/Liquid phase
49
What type of bond links monosaccharides together to form polysaccharides?
Answer
(B)
Glycosidic bond
50
What is the process by which glycogen is broken down into glucose?
Answer
(B)
Glycogenolysis