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Essential Biochemistry - Laboratory Investigation Techniques (Section 1)

1
What is the role of coenzymes?
Answer
(C)
They assist enzymes in catalyzing reactions
2
Which of the following is an example of a monosaccharide?
Answer
(C)
Glucose
3
What is the first step in radioimmunoassay?
Answer
(C)
Addition of labelled antigen and antibody
4
Which of these processes takes place in the mitochondria?
Answer
(B)
Krebs Cycle
5
What type of macromolecule is primarily responsible for the storage of genetic information?
Answer
(D)
Nucleic acids
6
Which of the following is NOT a lipid?
Answer
(D)
Polypeptides
7
Which of the following is a component of a colorimeter?
Answer
(D)
All of the above
8
Which of the following is a disadvantage of RIA?
Answer
(B)
Requires radioactive isotopes
9
In colorimetry the light absorbed is
Answer
(B)
Directly proportional to the concentration
10
What is measured by the enzyme activity assay?
Answer
(B)
product formation
11
Which of the following is used for chromatography separation?
Answer
(D)
All of the above
12
ELISA stands for?
Answer
(A)
Enzyme Linked Immunosorbent Assay
13
Which technique is mainly used to detect infectious diseases?
Answer
(A)
ELISA
14
Which of the following is a function of lipids?
Answer
A
B
D
15
Where does the Calvin cycle take place?
Answer
(B)
Chloroplast stroma
16
What are the advantages of using RIA?
Answer
A
D
17
The standard unit is in radioimmunoassay
Answer
(C)
µg/L
18
What is the process of synthesizing proteins from mRNA called?
Answer
(B)
Translation
19
What is the process by which a protein loses its shape and function due to disruption of its bonds?
Answer
(B)
Denaturation
20
What is a common function of the Krebs cycle?
Answer
(C)
ATP production
21
What is the process of converting amino acids into glucose called?
Answer
(C)
Gluconeogenesis
22
What are the components of a lipid?
Answer
B
C
D
23
Which of the following is a function of carbohydrates?
Answer
A
B
24
What is the primary role of cholesterol in animal cell membranes?
Answer
(B)
Structural support and regulating membrane fluidity
25
What principle is used in flame photometry?
Answer
B
C
26
Flame photometry is based on which principle?
Answer
(B)
Emission of light
27
What is the role of ribosomes?
Answer
(B)
Protein synthesis
28
In colorimetry, the color intensity is determined by
Answer
(A)
The concentration of the compound being measured
29
The color intensity measured by colorimetry is
Answer
(A)
Directly proportional to the concentration of the substance.
30
What is the product of glycolysis?
Answer
(B)
Pyruvate
31
What is a zymogen?
Answer
(A)
An inactive enzyme precursor
32
Where does the Krebs cycle take place in eukaryotic cells?
Answer
(C)
Mitochondria
33
The pH of the buffer used for separation of serum proteins by electrophoresis in agar gel is
Answer
(C)
8.6
34
What is the primary function of enzymes?
Answer
(C)
To catalyze reactions
35
Which of the following is a component of a cell membrane?
Answer
(C)
Phospholipids
36
Which of the following are monosaccharides?
Answer
A
B
37
What is the role of tRNA in protein synthesis?
Answer
(B)
Transporting amino acids
38
The heat of the flame is provided by
Answer
(D)
fuel and oxygen
39
What is the role of DNA polymerase?
Answer
(B)
Synthesizing DNA
40
What process produces the most ATP molecules?
Answer
(C)
Electron transport chain
41
The separation of serum proteins by electrophoresis is based on their
Answer
(B)
Charge
42
The following is the technique used to determine the concentration of sodium and potassium in biological fluids.
Answer
(A)
Flame photometry
43
Which of the following is a component of a nucleotide?
Answer
A
B
D
44
What is the role of the enzyme ligase?
Answer
(B)
To form bonds
45
The method is used to identify of major amino acids and drugs
Answer
(D)
All of the above
46
Which of the following techniques is used for the separation of amino acids, peptides, carbohydrates, hormones, drugs, vitamins and brain entities?
Answer
(D)
All of the above
47
Which of these is NOT a step in glycolysis?
Answer
(C)
Oxidative decarboxylation
48
Which of the following are types of enzyme inhibitors?
Answer
A
B
C
D
49
What is the mobile phase in paper chromatography?
Answer
(B)
The solvent
50
The most widely used method for determining the concentration of biochemical compounds is
Answer
(A)
Colorimetry