Biology for IGCSE & O level - Enzymes (Section 4 - No. 21)

Which of the following will denature an enzyme?
Freezing the enzyme
Boiling the enzyme
Changing the pH significantly
Adding a small amount of the substrate

Explanation

Both boiling and changing pH significantly can denature an enzyme. Freezing does not typically denature an enzyme, and adding a small amount of substrate will promote reaction.

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