Sequence: a gene is released from a human cell (2), then a gene is cut open by restriction enzymes (1), then a plasmid is cut open by the same restriction enzymes (3), then the gene is inserted into the plasmid, forming a recombinant plasmid(6), then the recombinant plasmid is inserted into the bacteria (5) and then Bacteria are grown in a fermenter to produce human protein (4).