Essential Biochemistry - Genetic Engineering (Section 2)

• 1

  Which of the following is not an application of genetic engineering?

  პასუხი

  (D)

  Development of new antibiotics
• 2

  What is the role of DNA ligase in recombinant DNA technology?

  პასუხი

  (B)

  Joining DNA fragments together.
• 3

  What is the purpose of a selectable marker in a cloning vector?

  პასუხი

  (A)

  To allow for the identification of cells that have taken up the vector.
• 4

  What is the key advantage of using plasmids as cloning vectors?

  პასუხი

  (A)

  They are small and easy to manipulate.
• 5

  Polymerase chain reaction (PCR) is a technique for

  პასუხი

  (A)

  Amplification of DNA in vitro
• 6

  Which of the following techniques is used to amplify DNA fragments?

  პასუხი

  (A)

  PCR
• 7

  What is the purpose of the extension step in PCR?

  პასუხი

  (C)

  To synthesize new DNA strands.
• 8

  Which of the following is NOT a common use of PCR?

  პასუხი

  (C)

  Genetically modifying an organism
• 9

  What is the role of a selectable marker in a cloning vector?

  პასუხი

  (B)

  To allow for the identification of cells that have taken up the vector.
• 10

  What is the function of a promoter in a cloning vector?

  პასუხი

  (A)

  To signal the start of transcription.
• 11

  What is the name of the process where foreign DNA is introduced into a bacterial cell?

  პასუხი

  (A)

  Transformation
• 12

  What is the primary difference between a genomic DNA library and a cDNA library?

  პასუხი

  (A)

  Genomic libraries contain all DNA sequences, while cDNA libraries contain only coding sequences.
• 13

  Which of the following is NOT a characteristic of a cloning vector?

  პასუხი

  (D)

  mRNA template
• 14

  Which one of the following enzyme is required for PCR?

  პასუხი

  (B)

  Taq polymerase
• 15

  What is the role of ligase in recombinant DNA technology?

  პასუხი

  (C)

  To join DNA fragments.
• 16

  Which of the following is not a step in recombinant DNA technology?

  პასუხი

  (D)

  Hybridization of the gene of interest with mRNA
• 17

  What is the function of a probe in Southern blotting?

  პასუხი

  (B)

  To identify a specific DNA sequence
• 18

  Which of the following are key components in the process of creating recombinant DNA?

  პასუხი

  A

  B

  C
• 19

  What type of analysis is used to detect the presence of specific DNA sequences in a sample?

  პასუხი

  (B)

  Southern blotting
• 20

  In genetic engineering, which of the following enzymes is used to join DNA fragments?

  პასუხი

  (B)

  DNA ligase
• 21

  The main purpose of using a cloning vector is to:

  პასუხი

  (D)

  All of the above.
• 22

  In gel electrophoresis, DNA fragments move toward which electrode?

  პასუხი

  (A)

  The positive electrode
• 23

  What is the principle behind the use of RFLP analysis?

  პასუხი

  (A)

  Differences in DNA sequence can lead to variations in the size of DNA fragments after digestion with restriction enzymes.
• 24

  Which of the following is an example of a commonly used restriction enzyme?

  პასუხი

  (B)

  EcoRI
• 25

  Which of the following is used to cut DNA at specific locations?

  პასუხი

  (C)

  Restriction enzyme
• 26

  Which technique is used to analyze RNA molecules?

  პასუხი

  (B)

  Northern blotting
• 27

  Restriction endonuclease has the following characteristics, except:

  პასუხი

  (D)

  Cut DNA randomly
• 28

  What does cDNA stand for?

  პასუხი

  (C)

  Complementary DNA
• 29

  Most commonly used cloning vectors are

  პასუხი

  (D)

  All of the above
• 30

  What is the role of a vector in recombinant DNA technology?

  პასუხი

  A

  C