Essential Biochemistry - Laboratory Investigation Techniques (Section 2)

  • 1
    The separation depends on the relative behaviour of molecules to
    Risposta
    (C)
    Both A and B
  • 2
    What is a cofactor?
    Risposta
    (B)
    A non-protein molecule that helps an enzyme
  • 3
    What is the function of a chaperone protein?
    Risposta
    (C)
    To assist in protein folding
  • 4
    The separation depends on the relative behaviour of the molecules in a mixture to associate more strongly with
    Risposta
    (B)
    the stationary phase
  • 5
    What is the primary function of mRNA?
    Risposta
    (A)
    To carry genetic information from DNA to ribosomes
  • 6
    Which of the following techniques can be used in small laboratories?
    Risposta
    (A)
    ELISA
  • 7
    Which of the following is a type of secondary structure in proteins?
    Risposta
    (C)
    Alpha helix
  • 8
    Which of the following is a purine base found in DNA and RNA?
    Risposta
    (C)
    Guanine
  • 9
    What type of reaction typically occurs when a molecule is broken down with the addition of water?
    Risposta
    (B)
    Hydrolysis
  • 10
    Which of the following can be detected by ELISA?
    Risposta
    A
    B
    C
  • 11
    Which molecule is the primary source of energy in cells?
    Risposta
    (A)
    Glucose
  • 12
    What is the role of ATP synthase?
    Risposta
    (B)
    To synthesize ATP
  • 13
    Which of the following is a characteristic of competitive enzyme inhibitors?
    Risposta
    (C)
    They increase the Km
  • 14
    What type of bond is formed between two amino acids?
    Risposta
    (B)
    Peptide
  • 15
    What is the role of the enzyme amylase?
    Risposta
    (C)
    Break down carbohydrates
  • 16
    The technique which depends on the antigen and the antibody is
    Risposta
    (C)
    Both A and B
  • 17
    What is the main function of the hormone insulin?
    Risposta
    (B)
    Decrease blood glucose levels
  • 18
    Which of the following is a technique used in ELISA?
    Risposta
    (D)
    Enzyme
  • 19
    What is the role of ATP in biological systems?
    Risposta
    (B)
    Energy transport
  • 20
    The application of RIA and ELISA is
    Risposta
    (D)
    All of the above
  • 21
    Which of the following is used for clinical application of electrophoresis?
    Risposta
    (D)
    All of the above
  • 22
    Which of the following molecules is a sterol?
    Risposta
    (B)
    Cholesterol
  • 23
    Which of the following is used as a label in ELISA?
    Risposta
    (D)
    Enzyme
  • 24
    What type of bond links amino acids together in a protein?
    Risposta
    (B)
    Peptide bond
  • 25
    Which of the following are the basic units of a colorimeter?
    Risposta
    (A)
    A light source, a cuvette, and a photodetector
  • 26
    What molecule is the final electron acceptor in the electron transport chain?
    Risposta
    (B)
    Oxygen
  • 27
    In the clinical laboratory for the identification of major amino acids and drugs in urine or serum.
    Risposta
    (D)
    All of the above
  • 28
    In paper chromatography, the solvent system provides
    Risposta
    (A)
    The mobile phase
  • 29
    What is the most abundant type of lipid in cell membranes?
    Risposta
    (C)
    Phospholipids
  • 30
    For which analysis colorimetry is used in the hospital?
    Risposta
    (D)
    All of the above
  • 31
    The most used technique to identify or quantify the antigen or antibody is
    Risposta
    (D)
    All of the above
  • 32
    Which of the following is a product of the Krebs cycle?
    Risposta
    (C)
    ATP
  • 33
    Inhibition of a certain pathway may be due to?
    Risposta
    A
    B
    C
    D
  • 34
    What does 'deamination' refer to?
    Risposta
    (A)
    Removal of an amino group
  • 35
    In radioimmunoassay, the sample is
    Risposta
    (B)
    Labeled with a radioactive isotope
  • 36
    Which of the following is a pyrimidine base found in DNA?
    Risposta
    (C)
    Cytosine
  • 37
    The ratio of the distance travelled by the solute to the distance travelled by the solvent is represented by
    Risposta
    (C)
    Both A and B
  • 38
    Chromatography is a process for separation of components in a solution by differences in migration rate as the solution moves
    Risposta
    (A)
    Mobile phase
  • 39
    What type of enzyme is responsible for breaking down proteins?
    Risposta
    (C)
    Protease
  • 40
    Which of the following can be measured using colorimetry?
    Risposta
    A
    B
    D
  • 41
    The method is applicable for the separation of carbohydrates, proteins, peptides, sterol acids, vitamins, steroids, vaccines like fragile viruses and drugs.
    Risposta
    (B)
    HPLC
  • 42
    What is the basic structural unit of a carbohydrate?
    Risposta
    (C)
    Monosaccharide
  • 43
    The energy generated by the detector in flame photometry is measured by
    Risposta
    (A)
    Galvanometer
  • 44
    ELISA is a technique for determining the presence of
    Risposta
    (C)
    Both A and B
  • 45
    The principle of spectrophotometry is based on
    Risposta
    (A)
    Beer-Lambert's Law
  • 46
    What type of reaction is the breakdown of ATP to ADP and inorganic phosphate?
    Risposta
    (A)
    Hydrolysis
  • 47
    Which of the following is often used as the stationary phase in paper chromatography?
    Risposta
    (A)
    Filter paper
  • 48
    In paper chromatography, the relative of a sample are separated by differences in their relative solubilities between
    Risposta
    (B)
    Liquid/Liquid phase
  • 49
    What type of bond links monosaccharides together to form polysaccharides?
    Risposta
    (B)
    Glycosidic bond
  • 50
    What is the process by which glycogen is broken down into glucose?
    Risposta
    (B)
    Glycogenolysis