Essential Biochemistry - Genetic Engineering (Section 2 - No. 23)
What is the principle behind the use of RFLP analysis?
Differences in DNA sequence can lead to variations in the size of DNA fragments after digestion with restriction enzymes.
Specific DNA sequences are amplified using PCR.
DNA fragments are separated based on their charge.
Proteins are identified by their specific binding properties.
Explication
RFLP analysis relies on variations in DNA sequences that affect restriction enzyme cutting sites.