Essential Biochemistry - Genetic Engineering (Section 2)

• 1

  Which of the following is not an application of genetic engineering?

  پاسخ دهید

  (D)

  Development of new antibiotics
• 2

  What is the role of DNA ligase in recombinant DNA technology?

  پاسخ دهید

  (B)

  Joining DNA fragments together.
• 3

  What is the purpose of a selectable marker in a cloning vector?

  پاسخ دهید

  (A)

  To allow for the identification of cells that have taken up the vector.
• 4

  What is the key advantage of using plasmids as cloning vectors?

  پاسخ دهید

  (A)

  They are small and easy to manipulate.
• 5

  Polymerase chain reaction (PCR) is a technique for

  پاسخ دهید

  (A)

  Amplification of DNA in vitro
• 6

  Which of the following techniques is used to amplify DNA fragments?

  پاسخ دهید

  (A)

  PCR
• 7

  What is the purpose of the extension step in PCR?

  پاسخ دهید

  (C)

  To synthesize new DNA strands.
• 8

  Which of the following is NOT a common use of PCR?

  پاسخ دهید

  (C)

  Genetically modifying an organism
• 9

  What is the role of a selectable marker in a cloning vector?

  پاسخ دهید

  (B)

  To allow for the identification of cells that have taken up the vector.
• 10

  What is the function of a promoter in a cloning vector?

  پاسخ دهید

  (A)

  To signal the start of transcription.
• 11

  What is the name of the process where foreign DNA is introduced into a bacterial cell?

  پاسخ دهید

  (A)

  Transformation
• 12

  What is the primary difference between a genomic DNA library and a cDNA library?

  پاسخ دهید

  (A)

  Genomic libraries contain all DNA sequences, while cDNA libraries contain only coding sequences.
• 13

  Which of the following is NOT a characteristic of a cloning vector?

  پاسخ دهید

  (D)

  mRNA template
• 14

  Which one of the following enzyme is required for PCR?

  پاسخ دهید

  (B)

  Taq polymerase
• 15

  What is the role of ligase in recombinant DNA technology?

  پاسخ دهید

  (C)

  To join DNA fragments.
• 16

  Which of the following is not a step in recombinant DNA technology?

  پاسخ دهید

  (D)

  Hybridization of the gene of interest with mRNA
• 17

  What is the function of a probe in Southern blotting?

  پاسخ دهید

  (B)

  To identify a specific DNA sequence
• 18

  Which of the following are key components in the process of creating recombinant DNA?

  پاسخ دهید

  A

  B

  C
• 19

  What type of analysis is used to detect the presence of specific DNA sequences in a sample?

  پاسخ دهید

  (B)

  Southern blotting
• 20

  In genetic engineering, which of the following enzymes is used to join DNA fragments?

  پاسخ دهید

  (B)

  DNA ligase
• 21

  The main purpose of using a cloning vector is to:

  پاسخ دهید

  (D)

  All of the above.
• 22

  In gel electrophoresis, DNA fragments move toward which electrode?

  پاسخ دهید

  (A)

  The positive electrode
• 23

  What is the principle behind the use of RFLP analysis?

  پاسخ دهید

  (A)

  Differences in DNA sequence can lead to variations in the size of DNA fragments after digestion with restriction enzymes.
• 24

  Which of the following is an example of a commonly used restriction enzyme?

  پاسخ دهید

  (B)

  EcoRI
• 25

  Which of the following is used to cut DNA at specific locations?

  پاسخ دهید

  (C)

  Restriction enzyme
• 26

  Which technique is used to analyze RNA molecules?

  پاسخ دهید

  (B)

  Northern blotting
• 27

  Restriction endonuclease has the following characteristics, except:

  پاسخ دهید

  (D)

  Cut DNA randomly
• 28

  What does cDNA stand for?

  پاسخ دهید

  (C)

  Complementary DNA
• 29

  Most commonly used cloning vectors are

  پاسخ دهید

  (D)

  All of the above
• 30

  What is the role of a vector in recombinant DNA technology?

  پاسخ دهید

  A

  C