Essential Biochemistry - Genetic Engineering (Section 1)
1
A scientist wants to identify a specific gene within a large genome. Which technique would be most suitable?
2
What does the term 'transgenic' mean?
پاسخ دهید(A)
An organism with a modified genome
3
Which of the following is a benefit of using recombinant DNA technology?
4
What is an advantage of using PCR?
پاسخ دهید(C)
It can amplify a specific DNA sequence
5
Which technique is used to detect specific RNA molecules?
6
Which of the following is NOT required for PCR?
7
What process does a restriction enzyme perform?
8
What is the purpose of Taq polymerase in PCR?
پاسخ دهید(C)
To synthesize new DNA strands
9
What does RFLP stand for?
پاسخ دهید(B)
Restriction Fragment Length Polymorphism
10
Which blotting technique is used to analyze proteins?
11
پاسخ دهید(B)
A collection of cDNA clones representing the expressed genes in a cell
12
What is the role of a selectable marker in a cloning vector?
پاسخ دهید(B)
To identify cells that have successfully taken up the vector
13
Which is NOT a common application of recombinant DNA technology?
14
What is the primary purpose of using restriction enzymes in recombinant DNA technology?
پاسخ دهید(B)
To cut DNA at specific sequences
15
What is the main function of a cloning vector?
پاسخ دهید(B)
To amplify a DNA fragment
16
Cloning a technique developed for amplifying the quantity of DNA in vitro
17
Which techniques would be useful to test the presence of a specific DNA sequence?
18
What is the purpose of a selectable marker in a cloning vector?
پاسخ دهید(B)
To identify cells containing the vector
19
Restriction endonucleases are enzymes:
پاسخ دهید(B)
Which clears DNA at specific sequences
20
Restriction endonuclease has the following characteristics, except:
21
The process of joining of DNA fragments to form a hybrid molecule is called:
22
What is the function of DNA ligase?
23
What is the role of a primer in PCR?
پاسخ دهید(C)
To initiate DNA synthesis
24
Which of the following techniques is used to separate DNA fragments based on their size?
25
What is a main goal of genetic engineering?
پاسخ دهید(B)
To alter the genetic makeup of an organism
26
Which technique is used to amplify a specific DNA sequence?
27
Which one of the following is not an enzyme?
28
Most commonly used cloning vectors are:
29
Which of the following is a key component of a cloning vector?
30
What is the role of a selectable marker in a cloning vector?
پاسخ دهید(B)
To identify cells that have taken up the vector.
31
In recombinant DNA technology, what does the term 'vector' refer to?
پاسخ دهید(D)
The carrier molecule (e.g., plasmid) used to introduce foreign DNA into a host cell
32
Which of the following techniques can be used to detect the presence of a specific protein?
33
What is the process of introducing foreign DNA into a host cell called?
34
Which one of the following enzyme is required for PCR?
35
پاسخ دهید(A)
The use of genes to treat diseases
36
Which of the following is NOT a step in the process of creating recombinant DNA?
پاسخ دهید(D)
Using a ribosome to synthesize the protein
37
Southern blot is a technique used for analysis of
38
پاسخ دهید(C)
An extrachromosomal DNA molecule
39
What is the purpose of a DNA library?
40
What is the role of a promoter region in a gene?
پاسخ دهید(B)
To signal the start of transcription
41
Restriction endonucleases are enzymes
پاسخ دهید(B)
Which shares DNA at specific sequences
42
What is the primary goal of gene therapy?
پاسخ دهید(A)
To replace faulty genes with healthy genes
43
What is the role of the annealing step in PCR?
پاسخ دهید(B)
To allow primers to bind to the DNA template
44
Which enzyme is used to seal the gaps between DNA fragments during cloning?
45
Which of the following is a technique used for gene cloning?
46
What is a transgenic organism?
پاسخ دهید(A)
An organism that has a foreign gene introduced into its genome.
47
What is the purpose of gel electrophoresis in recombinant DNA technology?
پاسخ دهید(B)
To separate DNA fragments by size
48
What is the role of reverse transcriptase in cDNA library construction?
49
What is the role of the primer in PCR?
پاسخ دهید(B)
To provide a starting point for DNA polymerase
50
What is the name of the process by which a virus transfers genetic material to a bacterium?
